Effect of Paclitaxel for Breast Cancer Treatment

Effect of Paclitaxel for Breast crabby person TreatmentEffect of paclitaxel along with withnia sominiferia on lactate dehydrogenase enzyme activity changes in 7,12 di methyl group benz(a) anthracene ingestd detractor crab louse wistar ratsDr.N.Muninathan1*, Dr.P. Mohanalakshmi2,Amb beesha Kondam k., Dr. S. Malliga31* Department of Biochemistry, Meenakshi Medical College and Research Institute, Enathur,Kanchipuram 631552, Tamil Nadu, India.2- Department of Biochemistry, Sri Muthukumaran Medical College, Chennai3- Department of Biochemistry, ESIC Medical College, ChennaiAbstractAim The resolve of this study is to investigate the changes in the levels of lactate dehydogenase enzyme(LDH) activity and efficacy of combining of paclitaxel along with Withnia Sominiferia against breast genus Cancer in experimental wights. Breast crab louse is the commonest genus Cancer among women in all developed countries (except Japan) as well as in North Africa, South America, and southeastern and western Asia. While the incidence of breast cancer appears to be increasing, mortality rates argon now declining in at least some western countries. Breast cancer ranks third when some(prenominal) the sexes are considered together and is clearly a significant global public health problem. Design/Methods Breast cancer was induced in rats by 7, 12 Di methyl benz(a) anthracene (DMBA) at the dosage of 20mgs dissolved in 0.5ml sunflower oil and administered into experimental animals for 28 weeks. In this study, we demonstrated that combination of paclitaxel and withania somnifera revert the changes in the rats from lethal demigod of DMBA within 30 days. Results All the isoenzymes LDH1 LDH5 were observed in cancer bearing animals. Expression of these isoenzymes were nominate be reduced in paclitaxel and Withania somnifera handle animals. Conclusions The treatment with combination of paclitaxel and withania somnifera effectively reduced LDH enzyme activity levels. So, from the o btained results it is concluded that paclitaxel and withania somnifera is capable of restoring the breast architecture.Key words Withania somnifera, DMBA, Paclitaxel, LDH and Breast cancer. IntroductionBreast cancer ranks third when both the sexes are considered together (Parkin, 1999) and is clearly a significant global public health problem.There are nearly 8,00,000 new cases of breast cancer worldwide separately year .In approximately half of these patients, breast cancer will be the eventual cause of death. Incidence of breast cancer in Indian women is non as high as in western countries (Sinha et al., 2003) .It is the second most common cancer among women in south India. The age standardized rates divert from 22 to 28 per 1, 00,000 women (Sanghvi, 1998). Although the rates appear to be lower than those seen in developed countries, the burden of cancer in India is alarming.Worldwide incidences of breast cancer handle to rise and geographical variations in breast cancer incid ence indicate that environment factors contribute to overall risk (Millikan, 1995). Exposure to environmental carcinogens early in life is thought to be one of the first events in the development of breast cancer. Each year breast cancer is diagnosed in 910,000 women worldwide and 376,000 women transcend from the disease. Most of these cases are in industrial countries e.g., North America (180,000) and Europe (220,000).PAHs an important class of chemical carcinogens that are widespread in the ambient environment due to fossil fuel combustion for energy production, transportation and industry. DMBA, a potent PAH recognized as an initiator of both skin and colored cancer (Masaaki Miyata et al). The covalent binding of DMBA metabolites to DNA has been implicated as a critical step in the initiation phase of cancers.Paclitaxel (Taxol), a naturally occurring antineoplastic drug agent has shown great promise in the therapeutic management of certain human solid tumors particularly in met astatic breast cancer and malevolence involves skin, lung and refractory ovaries. It is the original member of the taxane group of anticancer drugs derived from the bark and needles of the pacific yew tree Taxus brevifolia. Paclitaxels antitumor activity was dish aerialovered in1960s during a wide scale 35,000 plants-screening program sponsored by the National Cancer Institute (NCI), USA.Withania is widely used in Ayurvedic medicine in India and in Unani and Middle Eastern conventional medicines, where it is highly regarded as a panacea, aphrodisiac, and rejuvenative. Withania sominifera (L). Dunal (Solanaceae) commonly called Ashwagandha (Sanskrit) is an Ayurvedic Indian medicinal plant, which has been widely used as a home remedy for several ailments.(Bhattacharya et al., 1997)The use of ashwagandha in Ayurvedic medicine extends back more than 3000 to 4000 years (Upton, 2000) (Agarwall et al., 1997). It has been widely extolled as a tonic, especially for emaciation in people o f all ages, including babies, and enhances the reproductive function of both men and women. It has also been used for inflammation, especially in antitumor, arthritic and rheumatic conditions, for asthma, and as a major tonic to counteract aging and promote new longevity (Dhuley., 1998).Materials And Methods1.1. Chemicals7,12 Dimethyl benz (a) anthracene and Withania somnifera were purchased from Sigma chemical company, USA. All the other chemicals used were of analytical grade.1.2. Animal care and housingFemale Wistar rats, 6-8 weeks of age and weighing 150-200g, were used. The animals were procured from Central Animal residence Block, Meenakshi Medical College and Research institute, Kanchipuran, Tamil Nadu, India and maintained in a controlled environmental condition of temperature and humidity on alternatively 12 h light/ turned cycles. All animals were fed standard pellet diet (Gold Mohor rat feed, Ms.Hindustan Lever Ltd., Mumbai) and water ad libitum. This research work on w istar female rats was sanctioned and sanction by the Institutional Animal Ethical Committee (REG NO. 765/03/ca/CPCSEA).1.3. Experimental DesignThe animals were divided in to sextette groups of 6 animals each. Group I animals served as control, Group II, III, IV, V as animals do by with DMBA (20mg ) per animal in sunflower oil (0.5ml), three times a week for 28 weeks to induce skin cancer. After tumor induction Group III animals were treated with Paclitaxel (33mg/kg b.wt) once in a week for 4 weeks. Group IV animals were treated with Withania somnifera (250g/animal) for 30 days. Group V animals were treated with both Paclitaxel and Withania somnifera (as in group III and group IV). These were Group VI Control animals treated with paclitaxel and Withania somnifera for 28weeks plus 30 days.After the experimental period of 32 weeks, the animals were sacrificed by cervical decapitation.1.4. Biochemical analysisThe isoenzymes pattern of lactate dehydrogenase was separated by the mode of Dietz and Lubrano (1967).III. ResultsPlate 1 depicts the Isoenzyme pattern of lactate dehydrogenase in blood serum of control and experimental animals. All the isoenzymes LDH1 LDH5 were observed in cancer bearing (group II) animals. Expression of these isoenzymes were found be reduced in paclitaxel (group III) and Withania somnifera (group IV) treated animals. However a much significant reduction in the LDH isoenzyme expression pattern was observed in-group V animals treated with both paclitaxel and Withania somnifera.Lactate dehydrogenase (LDH) isoenzyme pattern in serum of control and experimental animalsLane 1ControlLane 2DMBA treatedLane 3Paclitaxel treatedLane 4Withania somnifera treatedLane 5Paclitaxel and Withania somnifera treated cancer bearing animalLane 6Paclitaxel and Withania somnifera treated control animalIV. DiscussionLactate dehydrogenase (LDH) is the most common clinical enzyme used in the cancer patients for prognostic purpose ( Invone et al., 1998). It has a n important role in germ kiosk functions and can predict responses to chemotherapy and the prospects of remission. Human cancer tissues typically exhibit 2-3 fold increases in glycolytic enzymes and LDH activity. Sandhya Mishra et al. (2004) have also reported the increased level of LDH in breast cancer patients.Anderson and Kovatik (1981) reported greater LDH activity in breast cancer conditions. There was a significant increase in LDH level in serum of cancer bearing Group II animals. This might be due to the membrane disruptions that caused the release of these enzymes from the cancer cells or the overproduction by the tumor cells (Helmes et al., 1998). The depute LDH activity may also have resulted from differences in the rate of synthesis, degradation or the excretion of the enzymes in the mammary cancer bearing animals. Schwartz (1973) has reported that among the isoenzymes LDH5 was six times greater than LDH1 in mammary tumor cells.Flavanoids have proved to possess antitumo r effect on various animal models (Ames et al., 1995). The biological and pharmacologic activity of Withania somnifera was associated with phenolic compounds mainly to flavanoids, aromatic acids and esters (Burdock, 1998 De catsro, 2001). Antioxidant activity of flavanoids may also be due to their structural features and its action on membrane (Saija et al., 1995 Mathur et al., 2003 Mohan et al., 2006). Paclitaxel being fat in flavanoid content possess antitumor and antiproliferative activities that stabilizes the membrane permeability and reduces the release of LDH.V. Statistical analysisFor statistical analysis, one way analysis of analysis of variance (ANOVA) was used, followed by the Newman-Keuls Multiple Comparison test.VI. ConclusionFrom the present study, the effect of Paclitaxel- Withania somnifera combination proved to be effective chemotherapeutic agent against DMBA induced Breast cancer in wistar rats compared to that of paclitaxel or Withania somnifera confirmed anal yzing the LDH isoenzymes levels in serum.ReferenceAgarwal, R., Diwanay, S., Patki, P., and Patwardhan, B ( 1999). Studies on immunomodulatory activity of Withania somnifera (Ashwagandha) extracts in experimental immune inflammation. 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